LCGC-North America-06-01-2021 | LCGC North America

Tools for Functional Assessment of Biotherapeutics

June 01, 2021

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We present the main analytical techniques for performing functional characterization of biotherapeutic products. Such assessments are particularly critical for biosimilars, where analytical testing must ensure functional comparability with the innovator product.

Bioanalysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC–MS, Part 1: An Overview

June 01, 2021

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This is the first article in a four-part series exploring the quantitative assessment of drugs and their metabolites in biological fluids (such as blood, plasma, and urine) and tissue homogenates using liquid chromatography–mass spectrometry (LC–MS).

Effects of Water on Adsorbents in Porous Layer Open Tubular (PLOT) Column Gas Chromatography (GC)

June 01, 2021

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Solid adsorbent gas chromatography (GC) columns, such as porous layer open tubular (PLOT) columns, are the best option for GC analysis of C1–C5 hydrocarbons, but water can affect retention and selectivity. We review the effects of water for different types of PLOT columns, and explain how to prevent or remediate the problem.

Where Has My Efficiency Gone? Impacts of Extracolumn Peak Broadening on Performance, Part III: Tubing and Detectors

June 01, 2021

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In recent articles, we reviewed the basic concepts of extracolumn dispersion and how this phenomenon can impact the quality of an LC separation. We now specifically discuss the effects of dispersion that can occur due to tubing and detectors.