Advances in LC Column Technology

This comparison of various silica-based HILIC stationary phases with different types of coatings provides a better understanding of how stationary phase selectivity can be influenced.

When the unique shape-selective properties of PGC are appropriately identified, PGC stationary phases can be harnessed for fine separations of structurally related compounds.

Serial coupling of different column types can provide several important benefits to the chromatographer, including fine-tuning selectivity to separate complex mixtures.

Pressure-enhanced liquid chromatography (PE-LC) offers a new approach for improving selectivity for large molecule separations. Examples shown here include short oligonucleotides in ion-pairing reversed-phase (IP-RP) liquid chromatography and larger nucleic acids in ion-exchange (IEX) chromatography.