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Continued from last month, this month's column discusses specially designed columns for use in highly aqueous environments. Many of these columns also can be used as regular reversed-phase columns for a range of mobile phases.

Dolan discusses some of the challenges of separating small peaks from large ones and gives some pointers on how to obtain a separation.

Solid-phase extraction of acidic, basic, and neutral pharmaceutical compounds. (Phenomenex Inc.)

Chiral separation of drug enantiomers. (Chiral Technologies, Inc.)

Polymer analysis using HPLC with evaporative light-scattering detection. (Polymer Laboratories, Inc.)

Solid-phase extraction of basic drugs. (Argonaut Technologies)

Use of an SPE-based kit for amino acid sample preparation before GC analysis. (Phenomenex Inc.)

GC analysis of an industrial solvent mixture. (SGE, Incorporated)

Analysis of gelatins using SEC with multiangle light-scattering detection. (Wyatt Technology Corporation)

HPLC separation of herbicide enantiomers. (Regis Technologies, Inc.)

Reversed-phase LC analysis of sulfate–steroid conjugates. (ZirChrom Separations, Inc.)

Description of GC analysis with selected-ion monitoring MS detection and retention-time locking. (Agilent Technologies)

Polymer analysis using GC–MS and LC–MS. (Impact Analytical)

SPE and LC–MS analysis of terfenadine. (Waters Corp.)

Analysis of orange juice using HPLC with evaporative light-scattering detection. (Alltech Associates, Inc.)

HPLC analysis of proteins and peptides. (Phenomenex Inc.)

Synthetic fused-silica capillary tubing with light-guiding properties. (Polymicro Technologies, LLC)

SPE preparation of biological samples for LC–MS analysis. (Spark Holland B.V.)

Dissolution testing using HPLC and UV spectroscopy. (Waters Corporation)

Ion-exchange separation of hydrolyzed oligonucleotides. (ZirChrom Separations, Inc.)

Description of software for HPLC method development. (Waters Corporation)

GC analysis of the EPA 8270 semivolatiles mixture. (SGE, Incorporated)

Ion-exchange analysis of a humanized monoclonal antibody. (Dionex Corporation)

Analysis of star-branched polymers using SEC with multiangle light-scattering detection. (Wyatt Technology Corporation)

Protein sample preparation using filtration microplates. (Orochem Technologies)

Thin-layer chromatography analysis of steroids. (Analtech, Inc.)

Capillary GC analysis using microwave oven column heating. (Antek Instruments L.P.)

Anion-exchange analysis of glycoprotein monosaccharides. (Dionex Corporation)