Liquid chromatography–tandem mass spectrometry (LC–MS/MS) reveals distinct sphingolipid patterns in anorexia and obesity.
|Articles|March 1, 2003
- LCGC North America-03-01-2003
- Volume 21
- Issue 3
Affinity Selection Techniques for Proteomic Studies
Two solutions to the problem of obtaining quantitative information about protein expression are to couple two or more chromatographic modes to increase resolution and to use affinity selection techniques.
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Leon Niezen and Gert Desmet discuss uncertainty-driven LC method development, peak overlap, and moving from in silico to real data.
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Sam Whitmarsh explores how retention-time models handle interlaboratory data, novel chemotypes, unusual phases and ionisable compounds, and what labs need to do to build useful in-house models.
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