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With the introduction of a new dedicated Polyphenol Analyzer by Antec, sensitive analysis of polyphenols in food and beverages at low costs has become possible in routine.
Recently Antec introduced a new ALEXYS Analyzer specifically designed for the analysis of Polyphenols in food and beverages. The system is based on HPLC with electrochemical detection (HPLC-ECD) to assure highest detection sensitivity and reduced cost of operation and ownership.
Flavonoids and flavanoids are naturally occurring antioxidant compounds belonging to the phytopolyphenols. Resveratrol, quercetin, and other polyphenolic flavonoids have been reported to be cancer preventive agents and are thought to have therapeutic importance in cardiovascular disease. These polyphenolic substances are not only found in wine, but also green tea, chocolate, cocoa and several other food products.
A method is presented for the analysis of epicatechin, epigallocatechin, epigallocatechingallate, myricetin, quercetin, keampferol and cis- and trans-resveratrol. The ALEXYS Polyphenols Analyzer consists of a dual pump high pressure gradient system, a well plate autosampler (for 2 well plates of 96 or 384 wells) and a Decade II electrochemical detector (ECD). Gradient HPLC is most suitable for analyzing multiple polyphenols in complex matrices (e.g., wine, tea, chocolate, etc.). At 5µL injection volume a detection limit of 2 – 5 nM has been obtained routinely, which can be improved to 0.1 – 0.5 nM by increasing the injection volume to 100 µL. At 20 nM an RSD of 2% for peak heights and areas was found.