Gas chromatography/mass spectrometry (GC/MS) is integral to the analysis of semivolatile organic compounds (SVOCs) in environmental matrices. Recent pressure on the helium (He) supply has required organizations to actively investigate hydrogen (H2 ) carrier gas, but most GC/MS analyses have reduced sensitivity and hydrogenation or dechlorination in the sources. The Agilent HydroInert source retains the ability to analyze a wide calibration range (0.1 to 100 µg/mL) and meet the U.S. Environmental Protection Agency (EPA) method 8270 calibration criteria when using H2 carrier gas.
Using LC-MS/MS Analysis for PFAS Detection in Biological Samples and Serum
November 1st 2024Per- and polyfluoroalkyl substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications. Due to their persistent nature; prevalence in blood, food and environment; and emerging toxicity, there is a significant concern for their presence in the human body. Analysis is especially complicated due to background contamination in laboratory supplies and system. This paper will discuss sample preparation and LC-MS/MS analysis of an expanded panel of PFAS compounds in serum.
Robust Oligonucleotide Molecular Weight Confirmation via LC/MS
October 31st 2024Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.