ASMS 2021 Monday Morning Session: Top-Down Protein Analysis

This Monday morning oral session on top-down protein analysis will be held 8:30-10:30 am in Ballroom B. The session, chaired by Caroline DeHart of the Frederick National Laboratory for Cancer, and presided by Joe Cannon of Bristol Myers Squibb, includes six talks addressing a variety of approaches and applications.

Corrine Lutomski of the University of Oxford will kick off the session with a talk on “Connecting Proteoform Diversity to Receptor Function Using Infrared-Activation Native Top-down of Membrane G Protein-Coupled Receptors on the Thermo Orbitrap Eclipse.” Next, Qianjie Wang of Michigan State University will discuss top-down proteomics in plant biology, looking specifically at large-scale delineation of proteoforms in arabidopsis leaf and the chloroplast proteins.”

Kendall Johnson of Northeastern University will then give a talk on the use of capillary electrophoresis coupled to tandem mass spectrometry for top-down proteomic analysis of limited samples.

Comprehensive characterization of intact histones using tandem trapped ion mobility, electron capture dissociation, and time-of-flight TOF MS will be addressed next, by Kevin Jeanne Dit Fouque, of Florida International University.

In the fourth talk, Kevin Schug of the University of Texas at Arlington will discuss liquid-chromatography–triple quadrupole MS for intact proteins analysis from biological samples with solid-phase extraction as a universal sample pretreatment.

Rachel Franklin of Oregon State University will close out the session with a talk entited, “Elucidating Phosphorylation Patterns of the Serine–Arginine–Rich Domain of the SARS–CoV–2 Nucleocapsid Protein Using Genetically Encoded Phosphoserine Analogs and ECD Fragmentation.”