Automated Extraction and Analysis of Isomeric Cortisone and Prednisolone

April 16, 2016

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.