Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.
Recombinant monoclonal antibodies (MAbs) are a growing class oftherapeutic molecules. Early in the development of MAbs, a large numberof harvest cell culture (HCC) samples must be screened for immunoglobulinG (IgG) titer, aggregations, and charge variants. This study describes atwo-dimensional (2D) high performance liquid chromatography (HPLC)work ow for af nity, size-exclusion, and ion-exchange analysis.
New HPLC, MS, and CDS Products from 2024–2025: A Brief Review
June 5th 2025This review highlights the high performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products that were introduced from 2024 to 2025, summarizing the technical specifications of each product and highlighting significant user benefits.
Extracting Oxygen Heterocyclic Compounds Using Deep Eutectic Solvents
June 2nd 2025Oxygen heterocyclic compounds are notable components in citrus-scented creams, though quality control is important when using these compounds. Researchers recently created a workflow for extracting these substances using a deep eutectic solvent (DES) for microwave-assisted extraction (MAE).
The LCGC Blog: A Simplified Guide for Weighted Fitting and its Significance in Separation Science
June 2nd 2025In this month's edition of the LCGC Blog, discover how weighted least squares regression enhances quantitation accuracy in analytical chemistry, particularly for low-concentration analytes in HPLC.