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Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.
Recombinant monoclonal antibodies (MAbs) are a growing class oftherapeutic molecules. Early in the development of MAbs, a large numberof harvest cell culture (HCC) samples must be screened for immunoglobulinG (IgG) titer, aggregations, and charge variants. This study describes atwo-dimensional (2D) high performance liquid chromatography (HPLC)work ow for af nity, size-exclusion, and ion-exchange analysis.