Automated MAb Workflow: From Harvest Cell Culture to Intact Mass Analysis of Variants

May 17, 2013

The Column

The Column, The Column-05-17-2013, Volume 9, Issue 9

Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.

Recombinant monoclonal antibodies (MAbs) are a growing class oftherapeutic molecules. Early in the development of MAbs, a large numberof harvest cell culture (HCC) samples must be screened for immunoglobulinG (IgG) titer, aggregations, and charge variants. This study describes atwo-dimensional (2D) high performance liquid chromatography (HPLC)work ow for af nity, size-exclusion, and ion-exchange analysis.

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