The Benefits of Moving from Single to Multi-detector Size-exclusion Chromatography
The addition of extra detectors such as light scattering and intrinsic viscosity to a single-detector size-exclusion chromatography system or, better still, the use of an integrated multi-detector size-exclusion chromatography system can pay significant dividends in the breadth of sample information generated.
The addition of extra detectors such as light scattering and intrinsic viscosity to a single-detector size-exclusion chromatography system or, better still, the use of an integrated multi-detector size-exclusion chromatography system can pay significant dividends in the breadth of sample information generated. This leads to a greater understanding of molecular weight, structure and composition, and hence can shorten the time required for process optimization.
Molecular weight can be measured independently of sample retention volume, eliminating the issues of column calibration, and intrinsic viscosity provides a direct measure of sample structure. The addition of a second concentration detector allows compositional information to be obtained. This webcast will provide an overview of multi-detector technologies and how they can benefit applications such as synthetic polymers, polysaccharides and proteins.
Sponsored by: Malvern Instruments
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
GPCRs – The Biological Traffic Modulator: Chromatographic Analysis of Peptides in the GI Tract
January 20th 2025The G protein-coupled receptors (GPCR) are found throughout the entire body and have shown significance in the development of new therapeutic treatments. Isolation of seven classic GRPC peptides initiating in the GI tract highlights the benefits of using the polymer-based PRP-3, a reversed-phase resin. The covalent bonds found in the PRP-3 exhibit advantageous interactions between the biological π bonds found in the peptides and the available aromatic benzyl rings of the resin.
