Beyond C18 and Phenyl: Expanding Selectivity With Biphenyl Columns Built for UV and pH Robustness

Metal interactions can silently impact peak shape, recovery, and reproducibility—are they affecting your HPLC results? Join our expert-led webinar to learn how modern inert-column technologies help overcome these analytical barriers and improve confidence in your data.

Register Free: https://www.chromatographyonline.com/lcgc_w/biphenyl

Event Overview:

Biphenyl stationary phases are increasingly used to resolve separations that challenge conventional C18 and phenyl chemistries, particularly for aromatic analytes and positional isomers. However, traditional biphenyl columns can suffer from elevated UV-detected bleed, limited pH stability, and inconsistent batch performance.
This presentation shows how a trifunctionally bonded biphenyl phase on a hybrid ethylene-bridged particle delivers balanced hydrophobic and aromatic selectivity with stable performance from pH 1.5–10 and ≥10× lower UV bleed. Inert column hardware further improves recovery of metal-sensitive analytes. Pharmaceutical, bioanalytical, and food/environmental examples demonstrate robust, reproducible separations.

Key Learning Objectives

• Recognize limitations of traditional biphenyl phases affecting robustness and quantitation.
• Explain the trifunctional biphenyl design that enables balanced selectivity and wide pH stability.
• Apply biphenyl selectivity and inert hardware to improve recovery.

Who Should Attend
Pharma method developers, R&D method developers, and bioanalytical scientists looking to optimize and future-proof their analytical methods.

This webinar is designed for scientists, analysts, and lab managers working on vaccine, lipid nanoparticle (LNP), or liposome characterization, as well as professionals at CDMOs, CROs, and testing organizations looking to expand their analytical capabilities.

Speaker:

Chris Collins, Ph.D.
Principal Product Marketing Manager
Waters Corporation

Chris Collins, Ph.D., earned his Ph.D. in analytical chemistry from North Carolina State University in 2014. He has over 20 years of experience in the pharmaceutical industry with a focus on LC/MS method development. Collins started working at Waters Corporation in 2018 as a chemistry account manager for North Carolina and South Carolina. In 2023, he transitioned to the role of principal product marketing manager for ACQUITY UPLC columns and MaxPeak Premier Column technology.

Register Free: https://www.chromatographyonline.com/lcgc_w/biphenyl