Biotherapeutics and Their Impurities and Degradants: Structural Characterization and QC
This session includes presentations on biotherapeutics and their impurities, including protein pharmaceuticals, glycans, glycopeptides, antibody-drug conjugates and more.
This session includes presentations on biotherapeutics and their impurities, including protein pharmaceuticals, glycans, glycopeptides, antibody-drug conjugates and more.
The first presentation in the session will be given by Joshua S. Sharp of the University of Georgia (Athens, Georgia) and is titled “Rapid Conformational Analysis of Protein Pharmaceuticals by an Abbreviated Hydroxyl Radical Footprinting Method.” Sharp will describe an abbreviated hydroxyl radical footprinting (HRF) technique for the conformational comparison of therapeutic protein samples using hydroxyl radical protein chemistry and LC-MS analysis.
The next talk, to be delivered by Maria Lorna A. De Leoz of NIST (Gaithersburg, Maryland) and the University of Maryland (College Park, Maryland), is titled “Comprehensive Mass Spectral Reference Library of Glycans and Glycopeptides for Therapeutic Antibodies” and will discuss a high-quality and comprehensive mass spectral reference library that was developed for characterizing glycan heterogeneity and sites of glycosylation in therapeutic antibodies.
Rebecca Rose of Utrecht University (Utrecht, Netherlands) will present the next talk, “The Relationship Between Mutation, Structure and Glycosylation of IgG Antibodies Revealed Using Native MS and HDX-MS.” Rose’s presentation will discuss the analysis of binding affinity of antibody heavy chains by native MS, and of allosteric structural changes influencing glycosylation by HDX-MS.
The fourth presentation in the session, “Glycosylation Analysis of Therapeutic Antibodies in Serum Samples Using Microfluidic CD Platform and MALDI-MS,” will be delivered by Thuy Tran of Stockholm University (Stockholm, Sweden). The presentation will discuss a method for glycosylation analysis of antibodies in cell culture media using a microfluidic CD platform and MALDI-MS that was recently modified with immunoaffinity purification of antibodies using the target antigen and used to analyze glycans of commercial therapeutic antibodies incubated in serum.
The penultimate presentation will be given by Justin Sperry of Analytical R&D at Pfizer, Inc. (Chesterfield, Missouri) and is titled “Subunit LC–MS Methods for the Characterization of Biotherapeutics: Case Studies for Antibody-Drug Conjugates and CRM197 Using Ultrahigh-Resolution QTOF Mass Spectrometry.” The talk will discuss the development of novel subunit LC-MS methods for heightened characterization of ADCs and CRM197, respectively.
Finally, Shiaw-Lin Wu of Northeastern University (Boston, Massachusetts) will present “Determining Cystine Knot and Disulfide Linkages in Recombinant Human Arylsulfatase A Using Multi-enzyme and LC-MS/MS with Electron Transfer Dissociation Approaches.” This presentation will identify very difficult disulfide linkages in a cystine knot for use in therapeutic protein development.
Measuring Procyanidin Concentration in Wines Using UHPLC
January 24th 2025Researchers from the University of Bordeaux (Villenave d'Ornon, France) report the development and validation of a rapid and quantitative analytical method measuring crown procyanidin concentration in red and white wines using ultra-high performance liquid chromatography (UHPLC) coupled with a ultra-high performance liquid chromatography (Q-TOF) mass spectrometer.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Testing Solutions for Metals and PFAS in Water
January 22nd 2025When it comes to water analysis, it can be challenging for labs to keep up with ever-changing testing regulations while also executing time-efficient, accurate, and risk-mitigating workflows. To ensure the safety of our water, there are a host of national and international regulators such as the US Environmental Protection Agency (EPA), World Health Organization (WHO), and the European Union (EU) that demand stringent testing methods for drinking water and wastewater. Those methods often call for fast implementation and lengthy processes, as well as high sensitivity and reliable instrumentation. This paper explains how your ICP-MS, ICP-OES, and LC-MS-MS workflows can be optimized for compliance with the latest requirements for water testing set by regulations like US EPA methods 200.8, 6010, 6020, and 537.1, along with ISO 17294-2. It will discuss the challenges faced by regulatory labs to meet requirements and present field-proven tips and tricks for simplified implementation and maximized uptime.
