This session includes presentations on biotherapeutics and their impurities, including protein pharmaceuticals, glycans, glycopeptides, antibody-drug conjugates and more.
This session includes presentations on biotherapeutics and their impurities, including protein pharmaceuticals, glycans, glycopeptides, antibody-drug conjugates and more.
The first presentation in the session will be given by Joshua S. Sharp of the University of Georgia (Athens, Georgia) and is titled “Rapid Conformational Analysis of Protein Pharmaceuticals by an Abbreviated Hydroxyl Radical Footprinting Method.” Sharp will describe an abbreviated hydroxyl radical footprinting (HRF) technique for the conformational comparison of therapeutic protein samples using hydroxyl radical protein chemistry and LC-MS analysis.
The next talk, to be delivered by Maria Lorna A. De Leoz of NIST (Gaithersburg, Maryland) and the University of Maryland (College Park, Maryland), is titled “Comprehensive Mass Spectral Reference Library of Glycans and Glycopeptides for Therapeutic Antibodies” and will discuss a high-quality and comprehensive mass spectral reference library that was developed for characterizing glycan heterogeneity and sites of glycosylation in therapeutic antibodies.
Rebecca Rose of Utrecht University (Utrecht, Netherlands) will present the next talk, “The Relationship Between Mutation, Structure and Glycosylation of IgG Antibodies Revealed Using Native MS and HDX-MS.” Rose’s presentation will discuss the analysis of binding affinity of antibody heavy chains by native MS, and of allosteric structural changes influencing glycosylation by HDX-MS.
The fourth presentation in the session, “Glycosylation Analysis of Therapeutic Antibodies in Serum Samples Using Microfluidic CD Platform and MALDI-MS,” will be delivered by Thuy Tran of Stockholm University (Stockholm, Sweden). The presentation will discuss a method for glycosylation analysis of antibodies in cell culture media using a microfluidic CD platform and MALDI-MS that was recently modified with immunoaffinity purification of antibodies using the target antigen and used to analyze glycans of commercial therapeutic antibodies incubated in serum.
The penultimate presentation will be given by Justin Sperry of Analytical R&D at Pfizer, Inc. (Chesterfield, Missouri) and is titled “Subunit LC–MS Methods for the Characterization of Biotherapeutics: Case Studies for Antibody-Drug Conjugates and CRM197 Using Ultrahigh-Resolution QTOF Mass Spectrometry.” The talk will discuss the development of novel subunit LC-MS methods for heightened characterization of ADCs and CRM197, respectively.
Finally, Shiaw-Lin Wu of Northeastern University (Boston, Massachusetts) will present “Determining Cystine Knot and Disulfide Linkages in Recombinant Human Arylsulfatase A Using Multi-enzyme and LC-MS/MS with Electron Transfer Dissociation Approaches.” This presentation will identify very difficult disulfide linkages in a cystine knot for use in therapeutic protein development.
Inside the Laboratory: The Gionfriddo Group at the University at Buffalo
March 28th 2024In this edition of “Inside the Laboratory,” Emanuela Gionfriddo, PhD, an associate professor of chemistry at the University at Buffalo, discusses her group’s current research endeavors, including using solid-phase microextraction (SPME) coupled to liquid chromatography (LC) and gas chromatography (GC) to further understand the chemical relationship between environmental exposure and disease and elucidate micropollutants fate in the environment and biological systems.
Transferring Methods to Compact and Portable HPLC
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.