ChloroFiltr®: A Novel Sorbent for Chlorophyll Removal Using QuEChERS

The Application NotebookThe Application Notebook-02-01-2022
Volume 40
Issue S2

Spinach and other highly pigmented vegetables contain chlorophylls, carotenoids, xanthophylls, and anthocyanins. Chlorophylls have the greatest adverse effect on gas chromatography systems due to their non-volatile characteristics. This QuEChERS procedure uses ChloroFiltr to significantly reduce chlorophylls without sacrificing the recoveries of planar pesticides.


1. QuEChERS Extraction

a) Homogenize 500 g of spinach in a food processor for 1–2 min

b) Weigh 10 grams of homogenized spinach sample into 50 mL centrifuge tube

c) Spike with 100 μL of 50 ppm triphenyl phosphate* as internal standard (IS)

d) Add 10 mL of acetonitrile then shake for 1 min

e) Add contents of Mylar pouch ECQUUS2-MP then shake vigorously for 1 min

f) Centrifuge at 5,000 rpm for 5 min

g) Supernatant is ready for clean-up

*50 ppm TPP solution: Mix 50 μL of 5000 ppm TPP solution with 4.95 mL of MeCN

2. dSPE Clean-up

a) Transfer 1 mL of the extract to the 2 mL CUMPSGGC182CT (ChloroFiltr)dSPE microcentrifuge tube

b) Shake for 30 s

c) Centrifuge at 3,000 rpm for 5 min

d) Transfer 0.4 mL of the supernatant to a 2 mL autosampler vial

e) Sample is ready for LC–MS/MS analysis

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