ChloroFiltr®: A Novel Sorbent for Chlorophyll Removal Using QuEChERS

ChloroFiltr®: A Novel Sorbent for Chlorophyll Removal Using QuEChERS

The Application Notebook, The Application Notebook-02-01-2022, Volume 40, Issue S2

Spinach and other highly pigmented vegetables contain chlorophylls, carotenoids, xanthophylls, and anthocyanins. Chlorophylls have the greatest adverse effect on gas chromatography systems due to their non-volatile characteristics. This QuEChERS procedure uses ChloroFiltr to significantly reduce chlorophylls without sacrificing the recoveries of planar pesticides.


1. QuEChERS Extraction

a) Homogenize 500 g of spinach in a food processor for 1–2 min

b) Weigh 10 grams of homogenized spinach sample into 50 mL centrifuge tube

c) Spike with 100 μL of 50 ppm triphenyl phosphate* as internal standard (IS)

d) Add 10 mL of acetonitrile then shake for 1 min

e) Add contents of Mylar pouch ECQUUS2-MP then shake vigorously for 1 min

f) Centrifuge at 5,000 rpm for 5 min

g) Supernatant is ready for clean-up

*50 ppm TPP solution: Mix 50 μL of 5000 ppm TPP solution with 4.95 mL of MeCN

2. dSPE Clean-up

a) Transfer 1 mL of the extract to the 2 mL CUMPSGGC182CT (ChloroFiltr)dSPE microcentrifuge tube

b) Shake for 30 s

c) Centrifuge at 3,000 rpm for 5 min

d) Transfer 0.4 mL of the supernatant to a 2 mL autosampler vial

e) Sample is ready for LC–MS/MS analysis

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