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News|Videos|October 30, 2026
Conversations with Michael Dong: A Case Study of a High pH Separation
Author(s)Michael W. Dong
In this final part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor presents a case study of a high-pH separation.
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Michael Dong, editor of LCGC International's "Perspectives in HPLC" column, has launched a video series in 2026 called "HPLC in Pharmaceutical Analysis," which explores how the technique is used in the pharmaceutical field.
In celebration of National Chemistry Week at the end of October, ChromatographyOnline.com is pleased to share "Conversations with Michael Dong" this month, featuring highlights from the series. We encourage readers to watch the full series, a free and thorough educational introduction to HPLC for pharmaceutical laboratory professionals at every stage of their careers who want to brush up on the basics. No matter how much experience you have with the technique, you're sure to come away with useful tips for sharpening your skills.
In this installment, Dong presents a case study of a high-pH separation.
The entire video, “Why is it OK to Use a MP pH Close to Analyte’s pKa?” can be accessed at
Previous videos in this series can be accessed here:
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"Perspectives in HPLC" examines 5 long-held HPLC "rules" (mobile phase filtration, routine buffer use, avoiding pH near an analyte's pKa, defaulting to 4.6-mm columns, and relying on AI for early method development), showing with experimental evidence that each is conditional rather than universally true in modern small-molecule drug testing.
In this ninth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explores the reasons why mobile phase filtration is not needed.
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