Deep Proteome Coverage on HeLa Lysate Using a bioZen™ Fractionation Column for High pH (Basic Reversed-Phase) Fractionation in Combination with a bioZen Nano Peptide XB-C18 Column for Nano Flow LC–MS

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We demonstrate how to achieve deep proteome coverage on HeLa cell lysate using a high pH reversed-phase fractionation strategy in combination with nano flow LC–MS.