|Articles|September 9, 2019

Determination of Average DAR of an Antibody Drug Conjugate (ADC) Mimic using a bioZen™ 1.8 µm SEC-2 Column

This application note serves to highlight how native MS can be used to get intact mass for non-covalently linked large molecules, including cysteine linked ADCs


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Chromatography Detects Toxins in Tea Samples
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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.