Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.
Using LC-MS/MS Analysis for PFAS Detection in Biological Samples and Serum
November 1st 2024Per- and polyfluoroalkyl substances (PFAS) are exceptionally versatile chemicals and have seemingly endless applications. Due to their persistent nature; prevalence in blood, food and environment; and emerging toxicity, there is a significant concern for their presence in the human body. Analysis is especially complicated due to background contamination in laboratory supplies and system. This paper will discuss sample preparation and LC-MS/MS analysis of an expanded panel of PFAS compounds in serum.
Robust Oligonucleotide Molecular Weight Confirmation via LC/MS
October 31st 2024Oligonucleotides—therapeutic agents for difficult-to-treat diseases—rely on precise molecular weight confirmation to ensure quality during development. Experts from Agilent Technologies explore the challenges of liquid chromatography-mass spectrometry (LC/MS) analysis in oligonucleotide synthesis, as well as strategies for accurate deconvolution, method development, and optimal equipment selection.
Development of a Fully Scalable High Efficiency 5um Solid-Core Particle to Support HPLC Workflows
October 30th 2024In this work the development of two key attributes of the 5um CORTECS Columns is examined. First column efficiency is compared across CORTECS and other solid-core 5 μm columns. Next scalability from sub-2 μm to 5 μm particles is examined between the CORTECS Column lines and competitive column lines. It was found that CORTECS columns have higher efficiency compared to other solid-core columns and that CORTECS particles are fully scalable.