Green Tea

January 22, 2015

E-Separation Solutions

A QuEChERS-based ultrahigh-performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC–QqQ–MS–MS) method was used to to determine pesticides in nutraceutical products from green tea (Camellia sinensis), according to a study published in the journal Food Chemistry.1

Green tea is produced from the steamed fresh leaves of the Camellia sinensis plant. It is said to have a range of health benefits and is rich in antioxidants. Nutraceutical products are derived from food sources, such as green tea, which are said to provide extra health benefits.

The team performed extraction with acidified acetonitrile (acetic acid 1% [v/v]); no further clean-up steps were required. Approximately 100 pesticides in nutraceutical products were determined using UHPLC–QqQ–MS–MS. Recoveries ranged from 70% to 117% and relative standard deviation (RSD) was lower than 20% at concentration levels of 25 μg/kg, 50 μg/kg, and 100 μg/kg for intra-day precision and equal or lower than 25% for inter-day precision. Limits of quantification (LOQ) were equal or lower than 25 μg/kg. The total run time was 11 min.

The method was applied to commercial nutraceutical products, and detected acetamiprid at 56 μg/kg and carbendazim at 13 μg/kg in two samples.


1. Gerarddo Martinez-Dominguez, Antonio Jose Nieto-Garcia, Roberto Romero-Gonzalez, and Antonia Garrido Frenich, Food Chemistry177, 182–190 (2015).