A QuEChERS-based ultrahigh-performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC–QqQ–MS–MS) method was used to determine pesticides in nutraceutical products from green tea (Camellia sinensis),
Volume 11 Issue 2
Pages 2-6
A QuEChERS-based ultrahigh-performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC–QqQ–MS–MS) method was used to determine pesticides in nutraceutical products from green tea (Camellia sinensis), according to a study published in the journal Food Chemistry.1
Green tea is produced from the steamed fresh leaves of the Camellia sinensis plant. It is said to have a range of health benefits and is rich in antioxidants. Nutraceutical products are derived from food sources, such as green tea, which are said to provide extra health benefits.
The team performed extraction with acidified acetonitrile (acetic acid 1% [v/v]); no further clean-up steps were required. Approximately 100 pesticides in nutraceutical products were determined using UHPLC–QqQ–MS–MS. Recoveries ranged from 70% to 117% and relative standard deviation (RSD) was lower than 20% at concentration levels of 25 μg/kg, 50 μg/kg, and 100 μg/kg for intraâday precision and equal or lower than 25% for inter-day precision. Limits of quantification (LOQ) were equal or lower than 25 μg/kg. The total run time was 11 min.
The method was applied to commercial nutraceutical products, and detected acetamiprid at 56 μg/kg and carbendazim at 13 μg/kg in two samples. - K.M.
Reference
Gerarddo Martinez-Dominguez, Antonio Jose Nieto-Garcia, Roberto Romero-Gonzalez, and Antonia Garrido Frenich, Food Chemistry177, 182–190 (2015).
This article is from The Column. The full issue can be found here>>
Microseparations and Analysis Covered at HPLC 2024
July 23rd 2024At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Recent Developments in HPLC & UHPLC
June 5th 2024In the world of liquid chromatography, innovative strides in column technology continue to take place. We are also reminded that there is always more to learn about “well-known” methodologies, and our craft is continuously influenced by important social concerns.