HPLC 2009 Tuesday Morning


HPLC 2009, Day Two

Welcome to Day Two of LC•GC Europe’s HPLC 2009 Show Daily.

The Tuesday morning programme of HPLC 2009 begins with parallel sessions on Proteomics, Molecular Basis of Separations and Environmental Analysis chaired by Bill Hancock, Attila Felinger and Guowang Xu respectively.

The Proteomics slot starts with a seminar on a micro-proteomics study of genetically engineered mouse models by David Lubman followed by a discussion of novel separation and identification techniques in proteomics by Yukui Zhang. The application of Displacement Chromatography for bottom-up and top-down proteomics approaches is covered by Helmut Schluter and the session closes with a talk by Edward Nice on the potential of faecal proteomics to detect and monitor colorectal cancer.

The Environmental Analysis lectures start with a talk by Thomas Ternes on LC quadrupole–linear ion trap MS to identify polar transformation products from Iodinated X-Ray contrast media followed by a case study by Guibin Jiang on a novel method to detect a brominated flame retardent near a manufacturing plant in China. Jin-Ming Lin describes a novel method of analysing phenolic compounds in environmental water samples using molecularly imprinted micro-solid-phase extraction combined with HPLC. Jun Haginake closes this session with an illuminating talk on a highly selective method to analysis of non-steroidal anti-inflammatory drugs in water samples using restricted access media-molecularly imprinted polymers.

Klaus Albert discusses ways to improve the understanding of the properties and retention behaviour of chemically bonded stationary phases in the keynote lecture for the Molecular Basis of Separations slot. This is followed by a discussion on a new approach on the solvation processes in reversed-phase liquid chromatography by Boguslaw Buszweski and a talk on comparing immunosorbents and MIPs for the selective extraction of a target analyte from complex samples by SPE sorbents based on aptamers. The closing lecture in this session by Sergey Sergayev describes enantioseparation of symmetrical and non-symmetrical analogues of Trogers base.

The second round of parallel sessions on Tuesday morning are Multidimensional Separations and Column Coupling, chaired by Peter Schoenmakers, Metabolomics, with Katja Detttmer and Chiral and Food Analysis chaired by Vadim Davankov.

After a coffee break the Multidimensional Separations sessions begin. First is a talk on the optimization of two-dimensional HPLC by Georges Guichon, followed by a discussion by Frank Steiner on the optimum LC set-up to separate all peaks in the shortest possible time. Pavel Jandera discusses the perspectives and limitations of reversed-phase HILIC comprehensive LC×LC and Deirdrie Cabooter describes the use of the kinetic plot method to design single and coupled column systems.

Ian Wilson starts the Metabolomics section with a stimulating lecture on the role of hyphenation in metabolic profiling. The challenges of using LC–MS metabonomics to diagnose disease and predict disease development are discussed by Guowong Xu and Multi-target Quantitative Metabolic Profiling by HPLC–ESI-MS–MS is covered by Michael Lammerhofer. Theresa Koal closes the session with a talk on MS–MS-based targeted metabolomics in biological samples with — and without — chromatography.

The Chiral and Food Analysis lectures includes Vadim Davankov on the dual separation mechanism of enantiomers in capillary electrophoresis, Thomas Hoffman on tracing the taste compounds in foods by combining chromatography and analytical sensory tools, Pilar Franco on finding the best separation for enantiomeric mixtures and Melanie Vogel on the determination of pesticides in different food matrices using LC–MS–MS.

Poster sessions and vendor seminars are showcased during the lunchbreak.

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