HPLC 2017 Monday Afternoon Sessions
Session Title: Mechanisms of Mass Transport Phenomena (FUN 3)
Chairs: Pavel Jandera and Frederic Lynen
14:00 - Fabrice Gritti: Combining Solvent and Non-Uniform Temperature Gradients to Improve Peak Capacity in Microfluidic Separations
14:30 - Deirdre Cabooter: Experimental Methodologies for the Assessment of Individual Mass Transfer Phenomena Revisited
14:50 - Szabolcs Fekete: System Band Broadening and its Impact in Modern Size-Exclusion Chromatography of Proteins
15:10 - Julia Rybka: Surface Diffusion of Aromatic Hydrocarbon Analytes in Reversed-Phase Liquid Chromatography
Session Title: Separation – High-Resolution MS (HYP 3)
Chairs: Michal HolÄapek and Zoltán Takáts
14:00 - Alexander Makarov: Orbitrap Technology in LC/MS and GC/MS: On the Road to High-Resolution in Every Lab
14:30 - Ying Ge: Top–Down Proteomics of Large Proteins up to 223 kda Enabled by Serial Size Exclusion Chromatography Strategy
14:50 - Govert Somsen: Highly Selective Characterization of Intact Proteoforms by HILIC-MS
15:10 - Christian Lanshoeft: A Generic Hybrid LBA-LC-HRMS-Based Workflow for Multiplexed hIgG1 Quantification in Pre-Clinical Species Directly at the Intact Protein Level
Session Title: Sample Preparation and Automation (APP 3)
Chairs: Emily Hilder and Doo Soo Chung
14:00 - Janusz Pawliszyn: Why are Thin Coated Sampling/Extraction Devices with Biocompatible Sorbents Well Suited for Coupling of Biological Systems to Liquid Chromatography/Mass Spectrometry?
14:30 - Gongke Li: Novel Online Sample Preparation Media for Complicated Samples Analysis Coupling with High-Performance Liquid Chromatography
14:50 - Massimo Morbidelli: Isoform Enrichment by Recycling Chromatography
15:10 - Astrid Gjelstad: Does Parallel Artificial Liquid Membrane Extraction Match the Requirements for an Applicable and High-Throughput Sample Preparation Method from Biological Matrices?
Session Title: Proteomics (YOU 3)
Chairs: Norman Dovichi and Paige Malec
14:00 - Barry L. Karger: The Role of Separations in Protein and Proteomic Mass Spectrometric Analysis
14:40 - Adam Kecskemeti: Characterization of a Poly(dimethylsiloxane) Microfluidic Chip Containing Immobilized Trypsin for Rapid Protein Digestion
14:52 - Jana Zemenová: Novel Approach to Determine Lipidated Analogs of Prolactin-Releasing Peptide via LC-MS Using Monolithic Column
15:04 - Peng Yu: Trinity P1 Mixed Mode Chromatography Enables Mass Spectrometry Compatible Orthogonal Peptide Separation to Reversed Phase
15:16 - Elizaveta Solovyeva: Predictive Chromatography of Peptides for Optimized Fractionation in Bottom-Up Proteomics
Session Title: Stationary Phases Based on Inorganic Supports (FUN 4)
Chairs: Attila Felinger and Monika Dittman
16:30 - Joe Pesek: Aqueous Normal Phase Chromatography: The Reliable and Rugged Method for Polar Compound Analysis
17:00 - Frederic Lynen: Enhancing Peak Capacity in Temperature Responsive Liquid Chromatography
17:20 - Alla Chernobrovkina: Revealing the Trends in Selectivity Changes Altering the Functional Layer of Novel HILIC Stationary Phases
17:40 - Estrella Sanz Rodriguez: Porous Silica Layers Open Tubular Multi-Channel Capillaries as Micro Analytical Platforms for Separation and Extraction
Session Title: Multidimensional Chromatography and MS Coupling (HYP 4)
Chairs: Peter Schoenmakers and Mark Schure
16:30 - William Craig Byrdwell: Comprehensive Two-Dimensional Liquid Chromatography with Quadruple Parallel Mass Spectrometry, LC1MS2 x LC1MS2 = LC2MS4
17:00 - Dwight Stoll: Improving the Resolving Power and Detection Sensitivity of Two-Dimensional Liquid Chromatography Separations of Protein Digests
17:20 - Bert Wouters: Implementation of an Immobilised-Enzyme Microfluidic Reactor in an Integrated Multi-Dimensional Liquid Chromatography System
17:40 - C J Venkatramani: High Resolution Analysis of Linker Drugs Used in Antibody Drug Conjugates (ADC's) by 2D-LC-MS: Transition of 2D-LC-MS From Research to Main Stream Pharmaceutical Analysis
Session Title: Metabolomics (APP 4)
Chairs: Koji Otsuka and Rawi Ramautar
16:30 - Guowang Xu: Metabolomics: From Differential Metabolite Discovery to Functional Elucidation
17:00 - Liang Li: Comprehensive and Quantitative Profiling of the Human Blood Metabolome from One Microliter of Finger Blood
17:20 - Dajana Vuckovic: Improving Metabolite Coverage in Global Metabolomics Using Sequential Extraction
17:40 - Paige Malec: Derivatization Strategies for Targeted Metabolomics
Session Title: Sample Preparation (YOU 4)
Chairs: Janusz Pawliszyn and Gongke Li
16:30 - Boguslaw Buszewski: Similarity, Selectivity, and Specificity (3s) – Three Dimensional Factor Determining HPLC Resolution
17:10 - Andrew Quigley: Determination of Selected Fat Soluble Vitamins From Bovine Milk Using Dispersive Liquid-Liquid Microextraction Coupled with HPLC-UV Equipped with a Core-Shell Column
17:22 - Sara Tengattini: Development of an HPLC Bioreactor Based on Immobilized Enterokinase for the Cleavage of Fusion Proteins
17:34 - Michel Raetz: Automated Robotic Sample Preparation for Metabolomics with Hyphenation to LC-SWATH/MS Acquisition for Plasma and Liver Samples
17:46 - Deyber Arley Vargas Medina: Easy and Cheap Apparatus for the Off-Line Automatization of Miniaturized Sample Preparation Techniques
Modern HPLC Strategies: Improving Retention and Peak Shape for Basic Analytes
August 16th 2024In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.
Quantification of Amino Acids in Sweat Samples with HPLC
September 18th 2024A recent study investigated the significance of quantifying amino acids in minute sweat volumes using high performance liquid chromatography (HPLC) with fluorescence (FL) detection, offering a non-invasive and efficient way to screen for diseases by detecting amino acids in sweat, even with minimal sweat volumes.