HPLC 2017 Tuesday Morning Sessions
Session Title: Supercritical Fluid Chromatography (FUN 5)
Chairs: Davy Guillarme and Lucie Nováková
08:30 -Caroline West: Ions and SFC: An Improbable Match?
09:00 -Didier Thiébaut: Evaluation of Alternative Solvents in Normal Phase Liquid and Supercritical Fluid Chromatography for Lipid Classes Analysis
09:20 -Abhijit Tarafder: Modeling Retention Behavior Under Solvent Gradient Condition in SFC
09:40 -Charlene Muscat Galea: Optimization of Temperature and Back-Pressure in Drug-Impurity Profiling by SFC: A Response Surface Design Approach
Session Title: Electrodriven Separations–MS (HYP 5)
Chairs: Marja-Liisa Riekkola and Andras Guttman
08:30 -Jonathan Sweedler: Measuring Endogenous D-Amino Acids in the Brain - From Brain Regions and Individual Cells to Cellular Release - Using CE and LC
09:00 -Rawi Ramautar: Resolving Volume-Restricted Metabolomics Using Sheathless Capillary Electrophoresis-Mass Spectrometry
09:20 -Christian Neusüß: Mass Spectrometric Characterization of Impurities of an Antibody Separated by SDS-Capillary Sieving Electrophoresis Using CSE-CZE-MS
09:40 -Aran Paulus: Capillary Electrophoresis-Mass Spectrometry for Intact Mass Analysis of Antibodies and Antibody-Drug-Conjugates
Session Title: Chiral Separations (APP 5)
Chairs: Wolfgang Lindner and Alberto Cavazzini
08:30 -Bezhan Chankvetadze: Recent Trends in Application of Polysaccharide-Based Chiral Selectors for Liquid Phase Separation of Enantiomers
09:00 -Gerhard Scriba: Capillary Electrophoresis in the Determination of the Stereoisomeric Purity of Drugs
09:20 -Kenji Hamase: Three-Dimensional HPLC Analysis of Amino Acid Enantiomers in Complicated Real World Samples
09:40 -Tivadar Farkas: Further Studies on the HILIC Character of Polysaccharide-Based Chiral Stationary Phases in Polar Organic Separation Mode
Session Title: Metabolomics and Lipidomics (YOU 5)
Chairs: Thomas Hankemeier and Michael Witting
08:30 -Liang Li: High-Coverage Quantitative Metabolomics Using Chemical Isotope Labeling LC–MS
09:10 -Francesca Rigano: Use of a Novel Linear Retention Index System as Identification Tool in Liquid Chromatography: Applications in Lipidomics
09:22 -Evelyn Rampler: Simultaneous Non-Polar and Polar Lipid Analysis by Complementary Online Two-Dimensional Liquid Chromatography and High Resolution Mass Spectrometry
09:34 -Hanne Roberg-Larsen: Oxysterols in Cancer
09:46 -Tim Causon: Increasing Peak Capacity and Selectivity of LC–MS by Incorporation of Low-Field Drift-Tube Ion Mobility Separation
Session Title: Novel High-Efficient Separation Media (FUN 6)
Chairs: Andrew Alpert and Frank Steiner
10:30 -Mary Wirth: Protein HPLC with Polymer Bonded Phases on Silica
11:00 -Jelle De Vos: Microfluidic Chip Technology to Advance Multidimensional Liquid Chromatography
11:20 -Yi Chen: Long-Range Uniform Photonic Crystals for High Performance Separation
11:40 -Kenichi Nagase: Thermoresponsive-Ionic Block Copolymer Brush Modified Stationary Phase for Thermally-Modulated Proteins Separation
Session Title: Nanofluidic and Microfluidic Separations–MS (HYP 6)
Chairs: Robert T. Kennedy and Peter Willis
10:30 -Robert T. Kennedy: HPLC–MS Analysis of Small Samples at High Pressures
11:00 -Andrea Gargano: Online Nano 2DLC Meets Top-Down MS: WCX-HILIC/a×m/RPLC UVPD-HRMS Analysis of Histone Proteoforms
11:20 -Wim De Malsche: New Developments in Pillar Array Column Technology
11:40 -Rico Warias: Chip-Based Liquid Chromatography–Mass Spectrometry: An Integrated Tool to Study Catalysis at the Microscale
Session Title: Lipidomics (APP 6)
Chairs: Gerhard Liebisch and William Craig Byrdwell
10:30 -Markus R. Wenk: Natural Variation of Blood Plasma Lipids in Healthy Asian Individuals
11:00 -Huwei Liu: Lipidomic Analysis of Plasma in Patients with Lacunar Infarction Using Normal-Phase / Reversed-Phase 2D LC–MS/MS
11:20 -Miroslav Lísa: Lipidomic Analysis of Biological Samples: Comparison of Liquid Chromatography, Supercritical Fluid Chromatography and Direct Infusion Mass Spectrometry Methods
11:40 -Michael Witting: Ion Mobility as Additional Separation Dimension to Tackle the Complexity of the Caenorhabditis Elegans Lipidome
Session Title: Chiral and Achiral Stationary Phases (YOU 6)
Chairs: Bezhan Chankvetadze and Alla Chernobrovkina
10:30 -Wolfgang Lindner: Principles of Enantioseparations in LC: State of the Art, Quo Vadis
11:10 -Ravindra Hegade: Stationary Phase Optimized Selectivity Chiral Liquid Chromatography (SOS-CLC) as a Novel Perspective for the Separation of Stereoisomers
11:22 -Judyta Hejniak: Flavanones Enantioseparation by LC–ESI-MS/MS and its Application
11:34 -Magdalena Skoczylas: Multi-Parametric Characterization of Amino Acids- and Peptide-Silica Stationary Phases – A Column Selection for Separation Targets
11:46 -Romana Jarosova: Nitrogen-Incorporated Tetrahedral Amorphous Carbon Thin-Films – A New Electrode for the HPLC-EC of Biological and Environmental Analytes
High-Throughput 4D TIMS Method Accelerates Lipidomics Analysis
April 25th 2024Ultrahigh-pressure liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) had been previously proposed for untargeted lipidomics analysis, but this updated approach was reported by the authors to reduce run time to 4 min.