But I Can't Change the Method

In this final part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor presents a case study of a high-pH separation.

Liquid chromatography–tandem mass spectrometry (LC–MS/MS) shows how processing and storage shape toxins in Nigerian rice.

Ultrahigh-pressure liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF-MS) reveals lipid shifts in acne-prone oily skin of young women.

This week, Chromatography Online.com covered the looming chromatography expertise gap, HPLC myths, steroid and biopharmaceutical separations, AI in the laboratory, and new sample extraction tools.

Gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) link tobacco leaf chemistry to cigar taste and burning.

The model context protocol (MCP) layer lets AI run mzmine mass spectrometry workflows; data stays local.

Ultrahigh-performance liquid chromatography–high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) reveals a chemical imbalance behind alcohol-positive milk.

"Perspectives in HPLC" examines 5 long-held HPLC "rules" (mobile phase filtration, routine buffer use, avoiding pH near an analyte's pKa, defaulting to 4.6-mm columns, and relying on AI for early method development), showing with experimental evidence that each is conditional rather than universally true in modern small-molecule drug testing.