In this final part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor presents a case study of a high-pH separation.
|Articles|July 1, 2004
- LCGC Europe-07-01-2004
- Volume 17
- Issue 7
Using Second-Order Data in Chromatographic Analysis
Author(s)Ricard Boqué, Joan Ferre
The authors discuss zero-, first- and second-order data and describe the advantages of using second-order chromatographic data compared with the more traditional univariate or multivariate calibration. They demonstrate how its use can uncover much more information from chromatograms and spectra.
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