|Articles|October 31, 2019

Increase Sensitivity and Reliability for Biopharmaceutical Applications by Simple Improvements in Sample Preparation

• Improving sample preparation improves sensitivity and reliability • Using a high-capacity paramagnetic bead solution to clean up & isolate mAbs • Optimize clean-up of labeling reagent, salts, or surfactants pre-analysis


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.