Investigation of Separation Conditions and Prep Purification of GLP-1 Receptor Agonist Semaglutide

The article describes a comprehensive automated prep purification workflow targeting semaglutide, a GLP-1 receptor agonist, using dedicated method development software to automatically optimize a gradient profile and search for conditions that meet the specified separation criteria. This was followed by scale-up to purification scale and streamlined confirmation of the purities of collected fractions using benchtop MALDI-TOF MS.