LC Columns for Biomolecule Separations
Analytical and nano scale Thermo Scientificâ„¢ Accucoreâ„¢ 150-C18 and 150-C4 columns are rugged and easy to use. The columns are designed to improve protein and peptide assay robustness by enhancing peak capacity and run times at moderate operating pressures.
Analytical and nano scale Thermo Scientific™ Accucore™ 150-C18 and 150-C4 columns are rugged and easy to use. The columns are designed to improve protein and peptide assay robustness by enhancing peak capacity and run times at moderate operating pressures.
The 150 angstrom pore diameter of the solid core particles are designed to provide the optimal combination of retention time and resolution for peptides and proteins. The Accucore 150-C18 has been further optimized for biomolecules and protein digests through bonding of C18 ligands onto the porous outer layer of the particles. The low hydrophobicity C4 ligand used in the Accucore 150-C4 column enables fast protein separations.
Advanced bonding technology produces excellent peak shapes through high bonding coverage and minimized secondary interactions. The automated packing processes produce uniform, tightly-packed columns which are robust and reproducible.
ref=http://info1.thermoscientific.com/forms/CMD_Accucore150_E861?ce=E.12CMD.CC119.G038650.00&gp=MOA-Media%20Online%20Adv> Learn more about Accucore HPLC columns for biomolecule separations
Measuring Procyanidin Concentration in Wines Using UHPLC
January 24th 2025Researchers from the University of Bordeaux (Villenave d'Ornon, France) report the development and validation of a rapid and quantitative analytical method measuring crown procyanidin concentration in red and white wines using ultra-high performance liquid chromatography (UHPLC) coupled with a ultra-high performance liquid chromatography (Q-TOF) mass spectrometer.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Testing Solutions for Metals and PFAS in Water
January 22nd 2025When it comes to water analysis, it can be challenging for labs to keep up with ever-changing testing regulations while also executing time-efficient, accurate, and risk-mitigating workflows. To ensure the safety of our water, there are a host of national and international regulators such as the US Environmental Protection Agency (EPA), World Health Organization (WHO), and the European Union (EU) that demand stringent testing methods for drinking water and wastewater. Those methods often call for fast implementation and lengthy processes, as well as high sensitivity and reliable instrumentation. This paper explains how your ICP-MS, ICP-OES, and LC-MS-MS workflows can be optimized for compliance with the latest requirements for water testing set by regulations like US EPA methods 200.8, 6010, 6020, and 537.1, along with ISO 17294-2. It will discuss the challenges faced by regulatory labs to meet requirements and present field-proven tips and tricks for simplified implementation and maximized uptime.
