Assessing phosphate concentration may be necessary to ensure that no differences are observed with modulating concentration. This ensures proper sample recovery and demonstrates robustness of the method. In this application note, we review SEC chromatographic performance for trastuzumab and NIST mAb modulating concentration of potassium phosphate.
Analyze Charge Variants in Antibody-Based Therapeutics Using Cation Exchange Chromatography
July 18th 2024This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.
The Power of HPLC/SFC Control and AQbD in One Software
July 17th 2024Download this infographic for a quick overview of Shimadzu’s LabSolutions Method Development (MD) software, an effective and automated software solution for U/HPLC method development that uses analytical quality by design (AQbD) principles in its workflow.