Live from SFC 2011
This series of interviews with leading speakers at the SFC 2011 conference (July 20-22, New York, NY) captures the key issues, concerns, advances, and solutions in relation to supercritical fluid chromatography (SFC).
Larry Miller discusses the role of SFC at Amgen and how it fits in with HPLC, the limitations of the method, and the role of the SFC conference.
Add this Podcast feed to your media player
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.