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This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.
Analysis of Cas9 IVT mRNA Aggregates
This technical note demonstrates the SEC-based separation and quantification of Cas9 IVT mRNA (with a total length of approximately 4500 nucleotides) and its aggregates.
EGFP mRNA Aggregation by SEC
SEC data demonstrates mRNA aggregate analysis for vaccine stability. Optimal separation occurs at 100 mM NaCl and low flow rates, enhancing mRNA production quality.
Plasmid Linearization In-Process Control Testing
The data show that a separation between circular and linear plasmid DNA is possible and the performed linearization can be monitored using SEC-HPLC.
Separation of SS from Duplex Oligonucleotides
This application showcases the utility of SEC to resolve s.s. oligonucleotides from duplexes in mixtures of four oligonucleotide lengths (21, 40, 80, and 100mers).
PEGylated Antibody Fragment Analysis with SEC-MALS (June 2024)
AAV Aggregate Analysis Mobile Phase Robustness
Biozen dSEC-7 accurately resolves AAV serotypes 5, 8 and 9 monomer from aggregates in under 10 minutes with consistent results across different mobile phase conditions.