|Events|June 5, 2023

Quantitative Analysis of 25-hydroxyvitamin D by LCMS

Comparison of analytical methods for quantification of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 on a Thermo Scientific™ TSQ Altis™ MD mass spectrometer


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
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