September Ezine Table I

This application note demonstrates the superior resolution and recovery of Agilent AdvanceBio SEC 500 Å columns when compared with competitor products for AAVs.

Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.

This application note demonstrates a liquid chromatography/quadrupole time-of-flight mass spectrometer (LC/Q-TOF MS) workflow for the identification and characterization of therapeutic peptides.

Spanish scientists recently tested flow injection analysis–mass spectrometry (FIA–MS) and its effectiveness in detecting fraudulent substances in Coleus forskohlii food supplements.

An optimized liquid chromatography (LC) method offers improved impurity separation, no batch reproducibility issues, and is unaffected by changes in gradient delay volume settings.