An understanding of analyte hydrodynamic radius (Rh), as well as size exclusion column pore size and distribution, are all critical in the understanding of how an analyte will behave by SEC. In this white paper, we will explore several protein standards with known hydrodynamic radii, and how this can be used to determine ideal size exclusion conditions for optimal method performance.
Analyze Charge Variants in Antibody-Based Therapeutics Using Cation Exchange Chromatography
July 18th 2024This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.
The Power of HPLC/SFC Control and AQbD in One Software
July 17th 2024Download this infographic for a quick overview of Shimadzu’s LabSolutions Method Development (MD) software, an effective and automated software solution for U/HPLC method development that uses analytical quality by design (AQbD) principles in its workflow.