The authors propose using water superheated to 100–240 ºC as an alternative liquid eluent for reversed-phase high performance liquid chromatography (HPLC) to overcome many of the problems of toxicity, flammability, and cost associated with organic modifiers. Superheated water has been demonstrated as an eluent with a number of stationary phase materials, including poly(styrene-divinylbenzene) and porous graphitic carbon both isothermally and in thermal gradient mode. It is compatible with conventional HPLC spectroscopic detectors and also can be used with flame ionization, nuclear magnetic resonance spectroscopy, and mass spectrometry detectors. The authors provide examples of separation applications, including aromatic compounds, pharmaceutical compounds, and vitamins.
LCGC 17(10), 938–945 (1999)Microseparations and Analysis Covered at HPLC 2024
July 23rd 2024At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Automated LC Method Development: An HTC-18 Interview with Bob Pirok
July 23rd 2024At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to this year’s winner of the 2024 HTC-18 Innovation Award, Bob Pirok from the University of Amsterdam, about his innovative research on automated LC method development.