Superheated Water: A New Look at Chromatographic Eluents for Reversed-Phase HPLC
The authors propose using water superheated to 100–240 ºC as an alternative liquid eluent for reversed-phase high performance liquid chromatography (HPLC) to overcome many of the problems of toxicity, flammability, and cost associated with organic modifiers. Superheated water has been demonstrated as an eluent with a number of stationary phase materials, including poly(styrene-divinylbenzene) and porous graphitic carbon both isothermally and in thermal gradient mode. It is compatible with conventional HPLC spectroscopic detectors and also can be used with flame ionization, nuclear magnetic resonance spectroscopy, and mass spectrometry detectors. The authors provide examples of separation applications, including aromatic compounds, pharmaceutical compounds, and vitamins.
LCGC 17(10), 938–945 (1999)
Investigating PFAS in Plastic Food Storage Bags Using LC–MS/MS
May 8th 2024Yelena Sapozhnikova from the United States Department of Agriculture spoke to The Column about her innovative research investigating PFAS in plastic food storage bags using targeted and non-targeted liquid chromatography–tandem mass spectrometry.
Vitamin D Determination Tested Using Liquid Chromatography–Tandem Mass Spectrometry
May 2nd 2024Scientists from Hangzhou Medical College recently recorded the effectiveness of novel quality control strategies for the determination of vitamin D using liquid chromatography–tandem mass spectrometry (LC–MS/MS).
