HPLC Detection and Evaluation of Chiral Compounds with a Laser-Based Chiroptical Detector

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LCGC North America, October 1999, Volume 17, Issue 10
Pages: 946–

The sensitivity and ruggedness of chiroptical detectors have improved considerably during the past decade. In this article, the authors examine more than 230 chiral compounds using the latest laser-based polarimetry detector for high performance liquid chromatography (HPLC). They also examine the relationship between optical rotation at the detector wavelength of 675 nm and the sodium D line. In addition, the authors consider the sensitivity, linear dynamic range, and effect of solvent composition on rotation and its general use as an HPLC detector for chiral compounds.