PharmTech LCGC Sciex Webinar 11.10
|Articles|September 1, 2009

Temperature Effects in GPC/SEC

Tips & Tricks: GPC/SEC

Temperature effects in GPC/SEC are very often misunderstood and discussed without keeping the specialties of GPC/SEC in mind. From a thermodynamic point of view the GPC/SEC separation mechanism is independent of the temperature. Therefore, there is no need to optimize the set-up for many applications with respect to temperature. However, the temperature influences the polymer solubility, the solvent viscosity and, therefore, the column performance. Consequently some applications require better knowledge about the influence of the temperature on these parameters.


Related to this article

Best of the Week: From Cancer Diagnostics to Smarter LC Method Development
This week, Chromatography Online’s coverage spanned lipidomics-based early cancer detection, career paths in separation science, machine learning and uncertainty-driven approaches to LC method development, new EU wastewater rules for pharmaceutical micropollutants, automated Separation Quality Factor (SQF) evaluation in chromatography data systems, and solutions to GLP-1 peptide analysis challenges.