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|Articles|September 3, 2021
The Effect of Column Internal Diameter for Robust Size Exclusion Chromatography
Author(s)Phenomenex, Inc.
The use of sub-2 µm particles for SEC have expanded analytical method for the characterization of biologics. This is especially noteworthy with monoclonal antibodies (mAbs), wherein methods developed on ultra high-performance SEC (UHP-SEC) columns can separate not only aggregates, but also fragment. This application note explores the effect of column internal diameter for robust size exclusion chromatography methods.
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