Tips & Tricks: GPC/SEC Is This Real? Unexpected Peak Shapes
GPC/SEC samples often show broad and/or multimodal peaks that are not baseline separated and look strange to users of other chromatographic techniques. However, although many weird looking peaks are possible in macromolecular characterization, some peaks are stranger than others and it is worth checking if the observed signal truly describes the sample.
GPC/SEC samples often show broad and/or multimodal peaks that are not baseline separated and look strange to users of other chromatographic techniques. However, although many weird looking peaks are possible in macromolecular characterization, some peaks are stranger than others and it is worth checking if the observed signal truly describes the sample. Therefore, it is good to know some tricks and hints that help identify measuring artefacts.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Investigating Plastic Contamination in Olive Oil with GC–MS/MS
January 17th 2025The objective of this study was to investigate contamination by 32 plasticizers in olive oil throughout its production and packaging process. Separation and detection were carried out by gas chromatography tandem mass spectrometry (GC–MS/MS), without the need for pre-concentration steps.
Top Execs from Agilent, Waters, and Bruker Take the Stage at J.P. Morgan Healthcare Conference
January 16th 2025The 43rd Annual Healthcare J.P. Morgan Healthcare Conference kicked off in San Francisco earlier this week. Here’s what top executives from Agilent, Bruker, and Waters, discussed during the event.
