Today’s Tutorials: Sample Prep, UHPLC, High-Pressures, and Why Small-Molecules Matter in Biopharma
Today’s free tutorial sessions at the HPLC 2016 conference will cover a range of topics, from trends in sample preparation and the use of high pressures, to fundamentals of ultrahigh pressure liquid chromatography (UHPLC), to the role of small-molecule analysis in biopharma.
Today’s free tutorial sessions will cover a range of topics, from trends in sample preparation and the use of high pressures, to fundamentals of ultrahigh pressure liquid chromatography (UHPLC), to the role of small-molecule analysis in biopharma.
The first tutorial of the day is at 9:20 am, when Ron Majors, column editor emeritus at LCGC, discusses new directions in sample preparation.
Then at 11:15 am, Monika Dittman of Agilent Technologies will discuss fundamental and instrumental aspects of UHPLC.
This afternoon, at 2:20 pm, Holly Shackman of Bristol-Myers Squibb will explain why small molecule analysis is important in biopharma.
The final tutorial of the day will be given by Torgny Fornsted of Karlstad University. At 4:40 pm he will present a session entitled, “Introduction to Advanced Separation Theory for Better Understanding of the Recent High Pressure Trend.”
All tutorials will be held in the Yerba Buena Ballroom, salons 5/6, on the B2 level.
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
