|Articles|September 9, 2019

Utilizing High Capacity bioZen™ MagBeads for the Immunocapture of Trastuzumab from Rat Plasma

Combining the efficient immunocapture and sample extraction of bioZen MagBeads, trastuzumab is isolated from rat plasma to yields greater selectivity relative to traditional ligand-binding assays.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.