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Optimizing Chromatographic Conditions for Protein Analysis with theACQUITY UPLC H-Class Bio System
Across the pharmaceutical industry, there is growing emphasis on the development of biopharmaceuticals as the next wave of drugs contributing to human health. In this podcast series, we’ll be discussing how chromatographic analyses of biopharmaceuticals, particularly proteins, compare to the more familiar assays of small molecule drugs and the benefits that UPLC® Technology can offer. The types of assays will be described and the basis for choosing them is considered. We’ll also discuss recent developments in ways to improve an analytical method.
Biopharmaceutical proteins are large, complicated molecules and demand multiple modes of analysis to ensure complete characterization of the sample. The choices in developing a separation method interact with detection, particularly in LC/MS. We will describe an experimental approach to optimizing this compromise.
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