Waters Podcast: HPLC vs. UPLC in practice


Everything you've ever wanted to know about UPLC: A practical guide to scientific and business achievement

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In this podcast series, we’ll be discussing how users can benefit from UPLC separations technology in their laboratories. We have heard all the hype about specifications from marketing, but what is it about this technology that, when in the hands of real users, allows them to achieve the scientific and business benefits that the chromatography community has proclaimed so widely in peer-reviewed journals, scientific meetings, and testimonials in the media

Through a variety of topics and scientific examples, this series will help you to gain a better understanding of UPLC technology, how to realize the benefits for your applications, and how to easily adopt UPLC technology for instant chromatographic success.

ACQUITY UPLC columns and systems are characterized by low dispersion and rugged operation at higher pressures and faster cycle times than HPLC. The decrease in volume is significant and has multiple ramifications and considerations to correctly scale HPLC flow rates, sample concentrations, and injection volumes. In this podcast, we’ll discuss the key differences between HPLC and UPLC separations, and the important things that current and prospective users should consider when migrating methods in order to achieve the very best in chromatographic performance.

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Toby Astill | Image Credit: © Thermo Fisher Scientific