Wednesday Morning Sessions

Wednesday’s programme begins with three parallel keynote lectures on Retention Mechanism, Biopharmaceuticals and Biomarkers chaired by Alberto Cavazzini, Douglas Westerlund and John Frenz repectively.

The Retention Mechanism session kicks off with a lecture by Nobuo Tanaka on the effect of pressure and solute retention on reversed-phase HPLC, followed by Boguslaw Buszweski offering new insights on residual silanols. Gert Desmet concludes this session with the use of computational fluid dynamics to establish and validate novel B-term diffusion expressions for packed bed and monolithic columns. The session concludes with Stefan Bruns on the physical reconstruction of packed beds and their morphological analysis.

Alois Jungbauer discusses the purification and characterization of large biomolecular assemblies by monolithic columns in a keynote lecture to start the Biopharmaceuticals session. This is followed by Maria Trusch on the analysis of proteomes by 2D–LC–MS. Jordan Plieskatt speaks on LC separation techniques for mass balance and characterization of recombinant vaccine candidates under cGMP and Sandra Grotefend closes the session with a lecture describing the application of various modes of high performance LC for protein analysis.

The Biomarkers session begins with a keynote lecture by Rainer Bischoff on the biomarker discovery for multiple sclerosis in cerebronospinal fluid by microfluidic HPLC–MS, followed by David Lubman discussing glycoproteomic markers of Glioblastoma of cancer stem cells. Milos Novotony continues this session with quantatative glycan profiling and oligosaccharide identification in cancer diagnosis and Martin Giera closes discussing novel derivatization strategies for LC–MS-based determination of biomarkers and cellular metabolites.

A session called Fundamental Aspects of Separations: Honouring Georges Guiochon chaired by Stephen Jacobson starts with an introduction on the importance of Georges Guiochon’s work by Franciso Farre Rius and Attila Felinger. Different approaches to complexity in stochastic theory of chromatography are discussed by Francesco Dondi, and Michel Martin then discusses the importance of Taylor dispersion analysis for size characterization of nanomaterials. Kanji Miyabe then describes moment equations for chromatography using superficially porous spherical particles.

Ion chromatography, Ionic Liquids, chaired by Nobuo Tanaka begins with Paul Haddad’s review of recent advances in ion chromatography. This is followed by Péter Hajós on integrated chemical systems for high performance ion chromatography using chelating, macrocyclic and dipolar ligands. María Celia García-Álvarez-Coque follows by talking about silanol suppression potency of alkyl-imidazolium ionic liquids on C18 stationary phases and Greg Dicinoski closes with a lecture on novel chromatographic systems to prevent acts of terrorism.

Christian Huber chairs the HPLC–MS session that begins with a keynote lecture by Gérard Hopfgartner on high-resolution liquid chromatography and fast acquisition mass spectrometry, followed by Christian Klampfl on HPLC coupled to direct analysis in real time mass spectrometry. Vilmos Kertész follows with the analysis of drugs and metabolites in whole-body thin tissue sections and Zsuzsanna Darula closes with method development for the selective isolation of secreted O-glycopeptides.

Poster sessions and vendor seminars take place during the lunch break.