The session chair for this Wednesday afternoon session is Emily Hilder of the University of South Australia. The session will be held in the Golden Gate Ballroom C, floor B2 level, beginning at 1:30 p.m.
The session chair for this Wednesday afternoon session is Emily Hilder of the University of South Australia. The session will be held in the Golden Gate Ballroom C, floor B2 level, beginning at 1:30 p.m.
This session’s first presentation is a keynote lection that will be given by Richard Smith of Pacific Northwest National Laboratory. The title of Smith’s presentation is “Ultrahigh Resolution and Ultrasensitive Ion Mobility Separations with MS Using Structures for Lossless Ion Manipulations.”
The next presentation in the session is a second keynote lecture titled “High Resolution and Ion Mobility Mass Spectrometry for Multidimensional Separations in -Omics” and will be presented by Gerard Hopfgartner of the University of Geneva.
The penultimate presentation of the session will be presented by Chunang (Christine) Gu of Genentech. The title of Gu’s presentation is “Structural Elucidation Challenges when 2D-HPLC Meets Mass Spectrometry.”
Lihua Zhang of the Dalian Institute of Chemical Physics at the Chinese Academy of Sciences will present the final talk in this Wednesday session. The presentation is titled “Quantitative Analysis of Proteomic Samples by Novel LC-MS/MS Based Methods.”
The session will be followed by Poster Session 4 in the Yerba Buena Ballroom Exhibition Hall, floor lower B2 level (poster presentations: Emerging Separation Techniques; Environmental and Energy-Related Analysis; Method Development and Automation; Protein Characterization, Modifications and Protein Binding; and Quality by Design [QbD] and Design of Experiment [DOE]).
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.