Application Notes: Size-Exclusion Chromatography (SEC)
SEC Columns for Large and Small Molecules
This application note introduces some new application data obtained by using FlexFire SEC that is adopted by newly developed silica gel.
Characterizing Lipid Nanoparticles (LNPs) for RNA Delivery
Field-flow fractionation coupled to multi-angle light scattering (FFF-MALS) is a powerful analytical tool that determines multiple CQAs of LNP-RNA nanoparticles.
Characterizing Branched Polymers by Light Scattering
SEC with multi-angle light scattering (SEC-MALS) or in conjunction with a viscometer (SEC-MALS-IV) effectively characterizes branching in polymers.
Understanding Antibody and Viral Glycoprotein Interactions (Sept 2022)
SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.
Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns (August 2022)
NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.
White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius (August 2022)
Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.
Improving Resolution for Size-Exclusion Chromatography Methods (August 2022)
Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column
Thermal Extraction of VOCs Using a Multi-ramp Method
This application note demonstrates a quick temperature ramp technique to improve the focusing of VOCs without a need for trapping.
Characterizing Vaccines with Light Scattering (August 2022)
Light scattering methods inform on essential properties and attributes of vaccines including size, aggregation, stability, interactions, composition, and conformation.
Characterization of Adeno-Associated Viral Vectors
Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.