Application Notes: Size-Exclusion Chromatography (SEC)
GPC/SEC Applications - Polymers, Biopolymers, Proteins
Tips & Tricks designed to support GPC/SEC users in their daily work, providing comprehensive overviews on different aspects of this powerful technique.
Saccharide and Polysaccharide Analysis (Aug 2023)
Robust, reliable GPC/SEC analysis of saccharides and polysaccharides can occur with very high resolution using Agilent SUPREMA columns with 3 and 5 µm particles.
PEGylated Antibody Fragment Analysis with SEC-MALS
Pore Size Selection for SEC of Oligonucleotides (June 2023)
Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).
Sensitive Quantification of AAVs/AAV Impurities
Determine down to 8x10^9 AAV capsids and separate impurities of AAV samples by separation using a mixed-bed SEC column & highly sensitive MALS detection.
SEC-MALS Characterization of a Drug-delivery Polymer
Learn the importance of light scattering detection to determine the true molecular weight distribution of a poly(glutamic acid)-based copolymer for drug-delivery.
Compound Separation with Size-Exclusion Chromatography
This application note introduces application data obtained by using FlexFire SEC Columns that have adopted newly developed superior silica.
Absolute Molecular Weight Determination of Natural Polymers Using ShodexTM OHpakTM LB-806M and MALS Detection
Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards.
New Shodex™ OHpak™ LB-800 HQ GFC Columns for Low Baseline Noise MALS Analysis
Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.
Shodex™ OHpak SB-806 HQ SEC Multi-Faceted Analysis of Exosome (EV) Combination of UV, Florescence, and Light Scattering Detectors
This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.