Application Notes: Size-Exclusion Chromatography (SEC)

Purification and SEC–MALS Analysis of VLP

March 26, 2021

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We investigated the purification and size-exclusion chromatography and light scattering analysis of virus-like particles.

Aggregates and Additives Analysis in Antibody Drugs

March 26, 2021

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This application note demonstrates the separation of IgG monomers and its aggregates, antibody metabolites, and other proteins.

LC–MS Analysis of Low MW Cations under HILIC mode

March 26, 2021

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LC–MS analysis of various low molecular weight cationic compounds were demonstrated using gradient formic acid and acetonitrile eluents.

LC–MS Analysis of Oligonucleotides v2

March 26, 2021

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Oligonucleotides were then analyzed using liquid chromatography with ultraviolet and mass spectrometry with a polymer-based diol column.

Analyzing multiple AAV CQAs with SEC-MALS

February 11, 2021

This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.

Characterization of Polymer Additives

October 09, 2020

Combining the benefits of the ACQUITY APC System with the ACQUITY UPLC Refractive Index (RI) Detector and the ACQUITY QDa Mass Detector yields laboratory efficiencies.

Column Screening for UPLC Separation

October 09, 2020

The selection of appropriate column chemistries to separate eighteen polymer additives are routinely monitored in extractables and leachables (E&L) workflows.

Aiding Lithium Ion Battery Electrolyte Design

October 09, 2020

A flexible analytical system was used in conjunction with a well-defined informatics workflow to elucidate degradation markers in Li-Ion battery electrolytes.

Two-Dimensional Liquid Chromatography for Polymer Applications

October 09, 2020

Combining the APC System with WinGPC UniChrom™ software allows researchers to increase the peak capacity of their research.

Accelerating Additive Manufacturing Breakthroughs

October 08, 2020

Collaborative efforts at Virginia Tech University drive better materials through polymer innovation and additive manufacturing.

SEC-MALS Analysis of Biotherapeutics

September 24, 2020

How UHP‑SEC‑MALS analysis using a UHPLC compatible multi-angle light scattering detector can be applied to determine absolute molecular weight of recombinant proteins.

Characterizing Vaccines with Light Scattering

September 14, 2020

Learn how light scattering characterizes multiple biophysical attributes of different vaccine modalities in support of discovery, development, manufacture, and QC.

Analysis of Hyaluronic Acid by SEC-MALS-IV

March 19, 2020

Polymer Branching Analysis by SEC-MALS-IV

March 19, 2020

Troubleshooting LC Separations of Biomolecules, Part 1: Background, and the Meaning of Inertness

March 01, 2020

How do bioinert and biocompatible liquid chromatography (LC) systems and columns improve separations of biomolecules? How do I know when these systems are required for my separation?

Analysis of Oligonucleotides by SEC

February 17, 2020

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Analysis of Oligonucleotides by SEC

November 19, 2019

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This note demonstrates the ability of a TSKgel® UP-SW2000 SEC column to discriminate oligonucleotides differing by one base in length.

Quantifying CQAs of gene therapy vectors

October 22, 2019

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This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.

Characterization of Hydroxyethyl Starch Using Gel Permeation Chromatography

September 18, 2019

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In this note, the molecular weight averages and molecular weight distributions of two hydroxyethyl starch (HES) samples were successfully demonstrated.