Application Notes: Size-Exclusion Chromatography (SEC)

Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns

March 11, 2022

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NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

SEC–High Resolution Mass Spectrometry (HRMS) for IdeZ Digested Monoclonal Antibody

March 11, 2022

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SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.

Method Optimization for the Analysis of Monoclonal Antibodies by Size-Exclusion Chromatography

February 16, 2022

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This technical report covers how a column packed with small particle material can be used to optimize an analytical method for monoclonal antibody aggregates.

Headspace Sampling for Flavour Profiling of Foods and Beverages

February 16, 2022

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Using high-capacity sorptive extraction with TD–GC–MS analysis for flavour profiling of cider and potato chips, contrasting unflavoured and flavoured-added products

Analysis of Protein Drugs Aggregation Using Size-Exclusion Chromatography

February 16, 2022

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This app note covers aggregate analysis using a Shimadzu Shim-pack™ BioDiol size-exclusion chromatography column with the Nexera XS inert UHPLC.

Conformational Properties of Hyperbranched Hetero-Polysaccharides v2

February 09, 2022

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This note discusses the characterization of conformational properties of hyperbranched macromolecules by combining SEC-MALS with differential viscometry.

Application Note: AAV Application Notebook

August 18, 2021

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AAV characterization and stability screening are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.

AAV Application Notebook

June 01, 2021

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Learn how AAVs are characterized for critical quality attributes and stability by multi-angle and dynamic light scattering.

Purification and SEC–MALS Analysis of VLP

March 26, 2021

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We investigated the purification and size-exclusion chromatography and light scattering analysis of virus-like particles.

Aggregates and Additives Analysis in Antibody Drugs

March 26, 2021

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This application note demonstrates the separation of IgG monomers and its aggregates, antibody metabolites, and other proteins.