Application Notes: Size-Exclusion Chromatography (SEC)

SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.

NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

This application note demonstrates a quick temperature ramp technique to improve the focusing of VOCs without a need for trapping.

Light scattering methods inform on essential properties and attributes of vaccines including size, aggregation, stability, interactions, composition, and conformation.

Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.

mAb and ADC aggregate analysis by SEC is introduced. Separation was improved by suppressing secondary interactions using high concentrations of corrosive salts.

Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.

The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.

Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.

Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity

Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).

This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.

NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.

This technical report covers how a column packed with small particle material can be used to optimize an analytical method for monoclonal antibody aggregates. 

Using high-capacity sorptive extraction with TD–GC–MS analysis for flavour profiling of cider and potato chips, contrasting unflavoured and flavoured-added products

This app note covers aggregate analysis using a Shimadzu Shim-pack™ BioDiol size-exclusion chromatography column with the Nexera XS inert UHPLC.  

This note discusses the characterization of conformational properties of hyperbranched macromolecules by combining SEC-MALS with differential viscometry.

AAV characterization and stability screening are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.

Learn how AAVs are characterized for critical quality attributes and stability by multi-angle and dynamic light scattering.

We investigated the purification and size-exclusion chromatography and light scattering analysis of virus-like particles.

This application note demonstrates the separation of IgG monomers and its aggregates, antibody metabolites, and other proteins.

LC–MS analysis of various low molecular weight cationic compounds were demonstrated using gradient formic acid and acetonitrile eluents.

Oligonucleotides were then analyzed using liquid chromatography with ultraviolet and mass spectrometry with a polymer-based diol column.

This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio. 

Combining the benefits of the ACQUITY APC System with the ACQUITY UPLC Refractive Index (RI) Detector and the ACQUITY QDa Mass Detector yields laboratory efficiencies.