Charged aerosol detection (CAD) is a powerful complement to ultraviolet (UV) absorbance and mass spectrometric (MS) detection for liquid chromatography (LC), particularly for analytes that have no UV chromophore, or do not ionize well by electrospray ionization. This article explores how to successfully use this technique.
LC users that are new to charged aerosol detection (CAD) will find behaviours that are different from ultraviolet (UV) detectors. This article discusses some of the most important of these behaviours and provides tips to shorten the path to usable data.
Charged aerosol detection is a powerful complement to UV and MS, but successful implementation requires understanding a few key factors, including response dependencies on temperature, nebulization process, analyte volatility, and mobile-phase composition.