Ronald E. Majors | Authors


Characterizing SEC Columns for the Investigation of Higher-Order Monoclonal Antibody Aggregates

With many new biopharmaceuticals now being developed, robust analytical methods are needed to ensure that these protein-based drugs are of high purity and safe with a minimum amount of side effects. Size-exclusion chromatography is an important technique in investigating purity and is useful to identify and monitor protein aggregation, which can have economic and immunogenicity effects. This article discusses those column parameters that are most important in the selection of the optimum phase for SEC separations.

Future Needs of HPLC and UHPLC Column Technology

In his final “Column Watch” article, Ron Majors looks into his crystal ball and discusses future needs in the area of HPLC/UHPLC column technology and related instrumentation. He looks at where current technology may be heading and makes a prediction that monolith-based columns may still have a rightful place in the HPLC/UHPLC laboratory .This article concludes his duration as a monthly columnist for LCGC.

Historical Developments in HPLC and UHPLC Column Technology: The Past 25 Years

During the course of my scientific career beginning in the 1960s, I have grown up with the birth of modern LC column technology, the refinements of the instrumentation, and the development of widespread application of this most powerful separation and analysis technique. In this installment, I would like to share with you some of my observations and experiences with the beginning, the growth period, and the maturation of HPLC columns, where I have focused nearly 33 years of writing for this magazine. I will explore some of the early column breakthroughs beginning with the development of large superficially porous particles (SPP), the porous irregular and spherical microparticulate particles, inorganic and organic polymeric monoliths and the rebirth of the current generation of SPP. In next month’s installment I will look into my crystal ball and see what the future of HPLC and UHPLC holds.