Shodex, Showa Denko America, Inc.

Here we introduce LC/MS analysis methods using the Shodex™ HILICpak™ VC-50 2D column for various cationic neurotransmitters.

The HILICpak™ VG-50 series columns are able to achieve high recovery rates of saccharides, organic acids, and amino acids.

Hydrophilic Interaction Liquid Chromatography (HILIC) can provide better separation for oligosaccharide analysis than Size Exclusion Chromatography (SEC) mode does.

This application demonstrated the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various sugars found in Japanese sake.

Webinar Date/Time: Wednesday, April 19, 2023

This application analyzes saccharides, organic acids, and amino acids using a Shodex™ HILICpak™ VG-50 2D column, with LC–MS alkaline gradient conditions.

This application shows how Shodex HILICpak columns can separate high-molecular weight oligosaccharides that proved difficult with preexisting HILIC columns.

This application shows howShodex HILICpak columns can separate high-molecular weight oligosaccharides that proved difficult with preexisting HILIC columns.

This application note shows how using the Shodex HILICpak VC-50 2D column with LC–MS provides highly sensitive analyses of various low molecular weight cationic compounds.

Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards.

Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.

This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.

When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered.

Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.

Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.

The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.

The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.

We investigated the purification and size-exclusion chromatography and light scattering analysis of virus-like particles.

This application note demonstrates the separation of IgG monomers and its aggregates, antibody metabolites, and other proteins.

LC–MS analysis of various low molecular weight cationic compounds were demonstrated using gradient formic acid and acetonitrile eluents.

Oligonucleotides were then analyzed using liquid chromatography with ultraviolet and mass spectrometry with a polymer-based diol column.

IgG, aggregates, antibody metabolites, and other proteins were analyzed on the PROTEIN LW-803 column

Oligonucleotides were separated in the order of polymerization without the use of ion-pair reagents.

LC–MS of various low MW cationic compounds analyzed using gradient under HILIC mode allows simultaneous analysis of multiple compounds with differences in cationic strengths.

Norovirus VLPs were purified from silkworms and investigated as a proof of concept.