OR WAIT null SECS
© 2022 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
Shodex™ has successfully developed the LB-800 columns which are suitable with MALS detection. The columns are well suited to provide signal at low concentration with low molecular weight standards.
Shodex™ has SB-800 HQ series for the analysis of water-soluble molecules and for the determination of molecular weight distribution.
This technical article introduces an example of multi-faceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex™ OHpak SB-806 HQ, with 3 different detectors.
When selecting a suitable analytical SEC column, not only the analytical range for the fractionation, but adsorption of the target compounds, which influences the quantification and yield, should also be considered.
Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.
Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.