OR WAIT null SECS
© 2022 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2022 MJH Life Sciences™ and Chromatography Online. All rights reserved.
Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.
Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.
The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.
The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.