YMC, Inc

Webinar Date/Time: Wednesday, April 19, 2023

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column, providing high intensities, recoveries, and great peak shapes.

In this application note high resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.

Amyloid β proteins are highly hydrophobic and can form aggregates. The analysis of 4 Aβ using YMC-Triart Bio C4 with larger pore and lower hydrophobicity is presented.

This work focused on scaling-down a monoclonal antibody SEC method, starting with a typical analytical-size SEC column (YMC-Pack Diol 5um, 300Å, 300x8.0mm) and ending with a small particle, shorter length, narrower-bore column (YMC-pack Diol 2um, 300Å, 150x4.6mm). The purpose of this experiment was to detail the differing performance characteristics exhibited by each column as physical properties were altered, one variable at a time.