YMC, Inc | Authors

In this application note high resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.

Amyloid β proteins are highly hydrophobic and can form aggregates. The analysis of 4 Aβ using YMC-Triart Bio C4 with larger pore and lower hydrophobicity is presented.

This work focused on scaling-down a monoclonal antibody SEC method, starting with a typical analytical-size SEC column (YMC-Pack Diol 5um, 300Å, 300x8.0mm) and ending with a small particle, shorter length, narrower-bore column (YMC-pack Diol 2um, 300Å, 150x4.6mm). The purpose of this experiment was to detail the differing performance characteristics exhibited by each column as physical properties were altered, one variable at a time.